LOCUS       40924_24643        5201 bp DNA     circular SYN 18-DEC-2018
DEFINITION  S. pombe expression vector pHIS3B DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     A novel series of vectors for chromosomal integration in fission 
            yeast
  JOURNAL   Biochem. Biophys. Res. Commun. 374 (2), 315-319 (2008)
  PUBMED    18634753
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     Fission yeast integration vector pHIS3B
  JOURNAL   Published Only in Database (2007)
REFERENCE   3  (bases 1 to 5201)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2007) Contact:Akihisa Matsuyama RIKEN, Chemical 
            Genetics Laboratory; Hirosawa 2-1, Wako, Saitama 351-0198, Japan URL
            :http://cgl.riken.go.jp/
REFERENCE   4  (bases 1 to 5201)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5201)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem. 
            Biophys. Res. Commun."; date: "2008"; volume: "374"; issue: "2"; 
            pages: "315-319"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Published 
            Only in Database (2007)"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (15-OCT-2007) Contact:Akihisa Matsuyama RIKEN, Chemical Genetics 
            Laboratory; Hirosawa 2-1, Wako, Saitama 351-0198, Japan URL 
            :http://cgl.riken.go.jp/"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     Fission yeast expression vector.
FEATURES             Location/Qualifiers
     source          1..5201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      21..208
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     misc_feature    242..808
                     /pseudo
                     /gene="his3"
                     /label=derived from Schizosaccharomyces pombe
                     /note="derived from Schizosaccharomyces pombe"
     CDS             242..808
                     /pseudo
                     /codon_start=1
                     /gene="his3"
                     /label=his3
                     /note="NotI site is created at the middle of this fragment 
                     (487-494)."
                     /translation="VLAAMKLLTP*FAFLVFLEKIRF*CVHLRTECIRSLQKSTMLRLL
                     RFSLNQISI*TSMLFAKHFLKIVPSKSFLLVPLVIQQRPLLNSRILRRFWSTLHGMESL
                     SSMRPILISLLPICLP*LLSMNIQTLLFAKPFRNLSV*QEFGKLI*AFKH*YYFFTNYD
                     V*YRILFDK*TYCYHYELIKGSL*H"
     gene            242..808
                     /pseudo
                     /gene="his3"
                     /label=his3
     promoter        816..1223
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        1230..1277
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             1296..1691
                     /codon_start=1
                     /label=BSD
                     /note="blasticidin S deaminase"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     terminator      1788..2035
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(2061..2077)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2290..2745
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3027..3131
                     /label=AmpR promoter
     CDS             3132..3989
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4163..4751
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5039..5060
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5075..5105
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5113..5129
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5137..5153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggcgcgccac ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag
       61 ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat
      121 tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagtatgagg
      181 tcgctcttat tgaccacacc tctaccggca gatccgctag ggataacagg gtaatatact
      241 agtgttggca gcgatgaaat tattgactcc ttaattcgca tttcttgtat tcctggaaaa
      301 gataagattt tgatgtgtcc accttcgtac ggaatgtata cggtctctgc aaaaatcaac
      361 gatgttgagg ttgttaaggt tctccttgaa ccagatttca atttgaacgt cgatgctatt
      421 tgcgaaacac tttctaaaga tagtgccatc aaagtctttt ttgcttgttc ccctggtaat
      481 ccaacagcgg ccgctcttaa actcgaggat attaagaaga ttttggagca ccctacatgg
      541 aatggaatcg ttgtcgtcga tgaggcctat attgatttct ctgctcccga tatgtctgcc
      601 ttaactcttg tcaatgaata tccaaacctt gctgtttgcc aaaccctttc gaaatctttc
      661 ggtctagcag gaattcggta agttaatcta agcttttaag cattaatatt atttctttac
      721 taactatgat gtttagtatc ggattttgtt tgacaagtaa acctattgct accattatga
      781 actcattaaa ggctccttat aacattagat ctagcccaca caccatagct tcaaaatgtt
      841 tctactcctt ttttactctt ccagattttc tcggactccg cgcatcgccg taccacttca
      901 aaacacccaa gcacagcata ctaaatttcc cctctttctt cctctagggt gtcgttaatt
      961 acccgtacta aaggtttgga aaagaaaaaa gagaccgcct cgtttctttt tcttcgtcga
     1021 aaaaggcaat aaaaattttt atcacgtttc tttttcttga aaattttttt ttttagtttt
     1081 tttctctttc gatgacctcc cattgatatt taagttaata aacggtcttc aatttctcaa
     1141 gtttcagttt catttttctt gttctattac aacttttttt acttcttgct cattagaaag
     1201 aaagcatagc aatctaatct aagggcggcg ttgacaatta atcatcggca tagtatatcg
     1261 gcatagtata atacgacaag gtgaggaact aaaccatggc caagcctttg tctcaagaag
     1321 aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact
     1381 acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat
     1441 atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg
     1501 cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc
     1561 cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga
     1621 aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg
     1681 tgtgggaggg ctaagcactt cgtggccgag gagcaggact gacacgtccg acgcggcccg
     1741 acgggtccga ggcctcggag atccgtcccc cttttccttt gtcgatatca tgtaattagt
     1801 tatgtcacgc ttacattcac gccctccccc cacatccgct ctaaccgaaa aggaaggagt
     1861 tagacaacct gaagtctagg tccctattta tttttttata gttatgttag tattaagaac
     1921 gttatttata tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg catgtaacat
     1981 tatactgaaa accttgcttg agaaggtttt gggacgctcg aaggctttaa tttgcaagct
     2041 gtttaaacga gctcgaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     2101 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     2161 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
     2221 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc
     2281 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     2341 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     2401 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     2461 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
     2521 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     2581 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg ggctattctt
     2641 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     2701 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca
     2761 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     2821 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     2881 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     2941 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     3001 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     3061 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     3121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     3181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     3241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     3301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     3361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     3421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     3481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     3541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     3601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     3661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     3721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     3781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     3841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     3901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     3961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     4021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     4081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     4141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     4201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     4261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     4321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     4681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     4801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     4861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     4921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     4981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     5041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     5101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     5161 acgccaagct tgcatgcctg caggtcgact ctagaggatc c
//
